lobitx.blogg.se

1. Detectx corticosterone enzyme immunoassay kit pdf software#
2. Detectx corticosterone enzyme immunoassay kit pdf free#

Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies.Ī cortisol-peroxidase conjugate is added to the wells.

Detectx corticosterone enzyme immunoassay kit pdf free#

Total cortisol is measured in extracted samples and in serum and plasma and free cortisol in saliva and urine.Ī cortisol standard is provided to generate a standard curve for the assay and all samples must be read off a user-generated standard curve. Moderate to severely hemolyzed samples should not be used in this kit. Samples containing particulates should be centrifuged prior to using. It has been validated for dried fecal extract samples. This assay has been validated for saliva, urine, serum and EDTA and heparin plasma and for tissue culture samples. The end user should evaluate recoveries of cortisol in other samples.

Detectx corticosterone enzyme immunoassay kit pdf software#

Software for converting raw relative optical density readings from the plate reader and carrying out four parameter logistic curve (4PLC) fitting.ĭiscover our best selling Cortisol ELISA KitĬortisol is identical across all species and this kit will measure cortisol from sources other than human. Repeater pipet with disposable tips capable of dispensing 25 μL, 50 μL and 100 μL.Ĭolorimetric 96 well microplate reader capable of reading optical density at 450 nm. Stop solution A 1M solution of hydrochloric acid. Wash buffer Concentrate A 20X concentrate that should be diluted with deionized or distilled water.

Dissociation reagent is to be used only with serum and Plasma samples. 28 mL or 55 mLĭissociation reagent 1 mL or 5 mL Allow to warm completely to room temperature prior to use. 3 mL or 13 mLĪssay buffer Concentrate A 5X concentrate that must be diluted with deionized or distilled water. 3 mL or 13 mLĭetectX® Cortisol Conjugate A cortisol-peroxidase conjugate in a special stabilizing solution. 125 μL or 625 μLĭetectX® Cortisol Antibody A mouse monoclonal antibody specific for cortisol. Whole Well Cortisol standard Cortisol at 32,000 pg/mL in a special stabilizing solution. It is already known that abnormal levels of cortisol are involved in Cushing's Syndrome and Addison's disease.Ĭlear Coated 96 Well Plate Each are coated with goat anti-mouse IgG. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer, depression, and schizophrenia. Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. It is often referred to as the 'stress hormone' as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. After a short incubation the color reaction is read at 450nm. The substrate reacts with the bound cortisol-peroxidase conjugate. After an 1 hour incubation the plate is washed and substrate is added. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol standard is provided to generate a standard curve for the assay. This kit measures total cortisol in extracted samples, serum and plasma and free cortisol in saliva and urine. The Cortisol Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples. Validated samples: Dried Fecal Extracts, Saliva, Urine, Serum, EDTA and Heparin Plasma and Tissue Culture Media The DetectX® Cortisol Immunoassay kit is designed to quantitatively measure cortisol present indried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples.įecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine